This article is an exam oriented Biochemistry question paper for D. Pharmacy students. It is useful for board exam preparation, revision, and practice for MSBTE, AKTU, BTEUP, RUHS and other state boards.

Biochemistry Practice Question Paper
Course: Diploma in Pharmacy
Time: 3 Hours
Maximum Marks: 80
Section 1: Long Answer Questions
Attempt any SIX of the following:
(6 × 5 = 30 Marks)
- Define the term enzyme. Enlist the factors affecting enzyme activity. Explain the effect of substrate concentration and temperature on the rate of enzyme-catalysed reaction.
- Explain beta-oxidation of unsaturated fatty acids. Write the energetics of palmitic acid.
- Enlist various qualitative tests of carbohydrates. Explain the principle, procedure, and significance of Molisch’s test and Benedict’s test.
- Give a schematic representation of the overall TCA cycle.
- Describe lipid profile tests and explain their clinical significance.
- Explain the secondary structure of proteins.
- Discuss the steps involved in glycolysis and explain its energetics.
Section 2: Short Answer Questions
Attempt any TEN of the following:
(10 × 3 = 30 Marks)
- Name the abnormal constituents of urine and the diseases associated with them.
- What are minerals? Explain the biochemical functions and deficiency disorders of calcium.
- Explain the electron transport chain (ETC) and oxidative phosphorylation.
- Explain the Watson and Crick model of DNA.
- Write the source, chemical nature, functions, and deficiency diseases of Vitamin A.
- What are fatty acids? Classify them based on chemical structure and nutritional requirements with examples.
- Define the following: a) Carbohydrates
b) Vitamins
c) Amino acids - Define dehydration. Write its causes and mention any one formula of ORS mixture.
- Enlist the tests used for assessment of kidney function. Explain GFR.
- What is anaemia? Explain the different types of anaemia.
- State the causes of: a) Phenylketonuria
b) Alkaptonuria
c) Ketoacidosis
Section 3: Objective Type Questions
Attempt ALL questions:
(20 × 1 = 20 Marks)
- Coenzyme A is a coenzyme form of Vitamin ______.
- Draw the structure of cholesterol.
- Write the full form of DNA and RNA.
- Define nucleotide.
- True or False: Prokaryotic cells possess a nucleus.
- Draw the structure of any one monosaccharide.
- Write the meaning of hyperkalemia and hypokalemia.
- Define biochemistry.
- Name the coenzyme derived from Vitamin B1.
- Which reaction identifies the presence of peptide bond?
- Give two examples of polysaccharides.
- Define nucleoside.
- What is the normal bilirubin level in adults?
- In conjugated proteins, when the prosthetic group is carbohydrate, it is known as ______.
- What is polycythemia vera?
- Give two examples of monosaccharides.
- ORS stands for ______.
- Define biotechnology.
- Which vitamin deficiency causes scurvy and night blindness?
- Define disaccharides with two examples.
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